Hemant Kumar Joshi
Biology
June 2022
Matrix metaloproteinases (MMPs) belong to the family of metzincin proteases with central roles in extracellular matrix (ECM) degradation and remodeling, as well as interactions with many growth factors and cytokines. Over expression of specific MMPs is responsible in many diseases such as cancer, neurodegenerative diseases and cardiovascular disease. MMPs have recently been the focus of attention as there are goals to develop therapeutics that can treat diseases related to MMP over expression. Upon activation and desalting, the pro domain is cleaved with an N-terminal His-tag, providing activated MMP-3cd for immediate use in countless in vitro applications. This method does not require expensive equipment or complex fusion proteins and describes the rapid production of recombinant human MMPs in bacteria. Antibacterial and anti-fungal activities of extracts of leaves of four different plants namely Madhumalati (Quisqualis indica), Aak (Calotropis procera), Latjira (Achyranthes aspera) and Tulsi (Ocimum sanctum) five bacteria (Bacillus subtilis, Enterobacter aerogenes) included in ten microorganisms. Codon Biotech Pvt Ltd, Noida for testing by well diffusion method against E. coli, Klebsiella pneumoniae, Pseudomonas aeruginosa) and five fungi (Alternaria pori, Aspergillus flavus, Aspergillus niger, Aspergillus aurizee, Penicillium chrysogenum) A study was conducted in the laboratory of Intravenous studies showed that methanol extract was more effective than aqueous extract. The extract of leaves of Madhumalati and Latjira was found to be more effective against fungal species. In contrast, extracts from leaves of Aak and Tulsi were found to be more effective against bacterial species.
426- 432
© Airo Journals 2021
