Sumana Samanta
Microbiology
March 2023
The primary aim of this study was to examine the growth of Listeria monocytogenes L56 (was to investigate the viability and proliferation of IMR isolates). Culture at 37°C Scanning electron microscopy (SEM) and motility media were able to identify morphological changes and motility induced by temperature stressors, respectively. Morphological changes and motility of Listeria monocytogenes in broth cultured in 300 ml TSB for 12 h at 37° C. for log phase and 19 h for stationary phase were measured at 2 h intervals. Longitudinal measurements of 180 cells were taken at 9,500x magnification using SEMafore for each temperature treatment. This study revealed that the morphology of Listeria monocytogenes cells changed significantly between exponential and stationary phases under stress conditions. At each of the three temperatures he studied, the stationary-phase cells were shorter and more spherical than the extended log-phase cells, while the extended-log phase cells were longer at 55°C, but at 28°C or 4°C. It did not stretch at °C. Loss of motility observed in stressed stationary-phase and unstressed log-phase cells indicates that mobility is critical for organismal survival under temperature stress
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