Dr. Sandip.N. Chavan
Botany
August 2025
Bioactive substances from medicinal plants are valuable in both traditional and modern health care systems, but habitat loss, overexploitation, and environmental conditions are reducing their genetic variety. Assessing and preserving diversity is essential for conservation and sustainable use. This study used molecular markers to examine the genetic diversity of three medicinal plants, Withania somnifera, Rauvolfia serpentina, and Ocimum sanctum. Leaf samples from several natural populations were used to isolate genomic DNA by CTAB and amplify it by RAPD, ISSR, and SSR. The amount of polymorphism was estimated using banding patterns, genetic diversity indices (Nei gene diversity and Shannon index), and Jaccards similarity coefficients were calculated before UPGMA cluster analysis. The results showed that O. sanctum was the most polymorphic, followed by W. somnifera and R. serpentine
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© Airo Journals 2021
