Sandhaya Sharma
Pharmacology
October 2025
The medicinal relevance of Syzygium cumini (L.) Skeels as a widely used drug in traditional medicine due to its varied therapeutic benefit has been underutilized, but its anticancer and anti-inflammatory effects are under-studied. This paper has explored the pharmacological impact of Syzygium cumini aqueous seed extract on human carcinoma cell lines with further focus on cytotoxicity, induction of apoptosis and alteration of cytokines. The cells that were cultured and exposed to different concentrations of extract were the HeLa (cervical carcinoma), MCF-7 (breast carcinoma) and normal fibroblast cells. Cytotoxicity was induced with the MTT assay, apoptosis with annexin V/PI staining, and anti-inflammatory effects by ELISA determination of IL-6 and TNF- 953 assays of LPS-stimulated cells. The extract exhibited the dose-dependent cytotoxicity of carcinoma cells and spared fibroblasts with the cell viability decreasing to approximately 2831 at 200 µg /mL. At higher doses the apoptotic index was found to increase dramatically reaching more than 50% which proved that the main mechanism of cytotoxicity was apoptosis. In addition, there was a significant inhibition of IL-6 and TNF-alpha levels, and this was an indication of high-grade anti-inflammation.
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© Airo Journals 2021
