Ankita Singh
Pharmacy
October 2025
The investigators in this work hope to one day be able to measure Sitagliptin in pharmaceutical formulations using a Reverse Phase High-Performance Liquid Chromatography (RP-HPLC) method. An essential medication for managing type 2 diabetes, sitagliptin acts as a selective inhibitor of the enzyme dipeptidyl peptidase-4 (DPP-4), which controls blood glucose levels. The need of precise quantification in providing consistent therapeutic effects and safety is highlighted by the research. Hypersil ODS C18 column and methanol/triethylamine mobile phase were optimized for RP-HPLC using high sensitivity UV detection at 237 nm. The method is suitable for routine pharmaceutical analyses since it is linear, exact, accurate, and resilient. The method's suitability for establishing a consistent Sitagliptin quality control is confirmed by its low limits of detection (LOD: 0.6773 µg/mL and LOQ: 2.1881/µg/mL), indicating a high level of sensitivity.
138- 154
© Airo Journals 2021
